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KMID : 0352720090330010008
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Journal of Ginseng Research
2009 Volume.33 No. 1 p.8 ~ p.12
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Rapid Determination of Ginsenosides Rb₁, Rf, and Rg₁ in Korean Ginseng Using HPLC
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Hong Hee-Do
Choi Sang-Yoon
Kim Young-Chan
Lee Young-Chul
Cho Chang-Won
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Abstract
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A simple gradient HPLC method for rapid determination of major ginsenosides (Rg₁ and Rb₁) and unique ginsenoside (Rf) of Korean ginseng (Panax ginseng C.A. Meyer) was developed. Within 50 min, three ginsenosides have been separated and identified on μ-Bondapak C18 column (3.9×300 ㎜, 10 ㎛) with gradient elution using water and acetonitrile as a mobile phase. The method was validated in terms of linearity, accuracy, and precision. The correlation coefficients (r²) for calibration curves of ginsenosides were over 0.9997. The developed HPLC method was successfully applied to the analysis of ginseng samples and the recoveries of ginsenosides were in the range of 101.1~115% with RSD＜3.2%. The developed method could be used for rapid evaluation of the ginsenosides Rg₁, Rb₁, and Rf.
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KEYWORD
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Korean ginseng, ginsenosides, HPLC-UV, method validation
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